abstract：:Rotavirus (RoV) and Norovirus (NoV) are the main causes of viral gastroenteritis. Currently, there is no validated multiplex real-time PCR that can detect and quantify RoV and NoV simultaneously. The aim of the study was to develop, validate, and internally control a multiplex one-step RT real-time PCR to detect and q...

journal_title：Journal of virological methods

authors： Dung TT,Phat VV,Nga TV,My PV,Duy PT,Campbell JI,Thuy CT,Hoang NV,Van Minh P,Le Phuc H,Tuyet PT,Vinh H,Kien DT,Huy Hle A,Vinh NT,Nga TT,Hau NT,Chinh NT,Thuong TC,Tuan HM,Simmons C,Farrar JJ,Baker S

更新日期：2013-01-01 00:00:00

abstract：:The SPF10 PCR targets a conserved 65bp region of the HPV L1 gene for broad-spectrum amplification. The LiPA assay allows subsequent genotyping of the HPV amplicons. This study aims to develop a SPF10 real-time PCR to achieve simultaneous amplification and detection of the HPV target. That way, LiPA analysis of the HPV...

journal_title：Journal of virological methods

authors： Micalessi MI,Boulet GA,Vorsters A,De Wit K,Jannes G,Mijs W,Ieven M,Van Damme P,Bogers JJ

更新日期：2013-01-01 00:00:00

abstract：:A recombinant hepatitis B surface antigen (HBsAg) has been produced in the yeast Saccharomyces cerevisiae and used as a vaccine against hepatitis B virus (HBV) infection. The present study aimed to optimize the extraction of recombinant virus-like particles (rVLPs) to develop a simple purification procedure based on g...

journal_title：Journal of virological methods

authors： Hadiji-Abbes N,Martin M,Benzina W,Karray-Hakim H,Gergely C,Gargouri A,Mokdad-Gargouri R

更新日期：2013-01-01 00:00:00

abstract：:In many countries phytosanitary regulations apply to Potato spindle tuber viroid, because it can cause serious diseases in potato and tomato crops. Other pospiviroids, some of which are distributed widely in ornamental crops, can cause similar diseases. Consequently, there is a need for a reliable and cost-effective g...

journal_title：Journal of virological methods

authors： Botermans M,van de Vossenberg BT,Verhoeven JT,Roenhorst JW,Hooftman M,Dekter R,Meekes ET

更新日期：2013-01-01 00:00:00

abstract：:Adenoviruses are highly efficient vectors for gene transfer into brain cells. Restricting transgene expression to specific cell types and maintaining long-term expression are major goals for adenoviral-mediated gene transfer in the central nervous system. Human adenovirus type 5 (Ad5) mediated transgene expression is ...

journal_title：Journal of virological methods

authors： Yan R,Zhang L,Zhang Q,Li J,Kang X,Wang H,Zhang J,Yang S,Yang X

更新日期：2012-12-01 00:00:00

abstract：:Viruses belonging to the Flaviviridae family are found and distributed in most of the tropical and sub-tropical regions of the world. The genus has more than 56 members, most of which cause clinical symptoms in humans. The clinical diagnosis of dengue requires laboratory confirmation because of the similarity of sympt...

journal_title：Journal of virological methods

authors： Honda ER,Zanchi F,Rios K,Lira E,DeusileneVieira,da Silva LH,De Paula SO

更新日期：2012-12-01 00:00:00

abstract：:Atypical bovine pestiviruses are related antigenically and phylogenetically to bovine viral diarrhea viruses (BVDV-1 and BVDV-2), and may cause the same clinical manifestations in animals. Glycoprotein E(rns) of an atypical bovine pestivirus Th/04_KhonKaen was produced in a baculovirus expression system and was purifi...

journal_title：Journal of virological methods

authors： Vijayaraghavan B,Xia H,Harimoorthy R,Liu L,Belák S

更新日期：2012-11-01 00:00:00

abstract：:A recombinant UL30 antigen-based single serum dilution enzyme linked immunosorbent assay (ELISA) was developed to measure specific antibody in the sera of ducks against duck enteritis virus (DEV). The partial UL30 gene of DEV was cloned, expressed, purified and tested for its diagnostic use by designing a single serum...

journal_title：Journal of virological methods

authors： Aravind S,Patil BR,Dey S,Mohan CM

更新日期：2012-11-01 00:00:00

abstract：:Adequate treatment of influenza requires identification of viral type as well as detection of mutation(s) conferring drug resistance. Reverse hybridization-based line probe assays (LiPA) can be performed using several probes immobilized on nitrocellulose, strips enabling LiPA to determine simultaneously viral subtypes...

journal_title：Journal of virological methods

authors： Miyoshi-Akiyama T,Akasaka Y,Oogane T,Kondo Y,Matsushita T,Funatogawa K,Kirikae T

更新日期：2012-11-01 00:00:00

abstract：:Standard methods for assessing the thermal stability of viruses can be time consuming and rather qualitative yet such data is a necessary requisite for vaccine formulation. In this study a novel plate-based thermal scanning assay for virus particle stability has been developed (PaSTRy: Particle Stability Thermal Relea...

journal_title：Journal of virological methods

authors： Walter TS,Ren J,Tuthill TJ,Rowlands DJ,Stuart DI,Fry EE

更新日期：2012-10-01 00:00:00

abstract：:In many mammals, viruses cause hepatitis. Despite many efforts a specific virus responsible for canine idiopathic hepatitis has not been identified. The discovery of a viral etiology in canine hepatitis will promote the development of specific drugs and vaccines for the treatment of idiopathic hepatitis in dogs. The o...

journal_title：Journal of virological methods

authors： van der Heijden M,de Vries M,van Steenbeek FG,Favier RP,Deijs M,Brinkhof B,Rothuizen J,van der Hoek L,Penning LC

更新日期：2012-10-01 00:00:00

abstract：:Real-time polymerase chain reaction (real-time PCR) is an accurate, rapid and reliable method that can be used for the detection and also for the quantitation of specific DNA molecules. It can be non-specific, with intercalating dyes (SYBR Green I dye) able to bind to any dsDNA, or specific with a probe (TaqMan), wher...

journal_title：Journal of virological methods

authors： Jamnikar Ciglenečki U,Toplak I

更新日期：2012-09-01 00:00:00

abstract：:HIV-1 integrase (HIV-1 IN), a well-validated antiviral drug target, catalyzes multistep reactions to incorporate viral DNA into the genome of the host cell; these include both a 3'-processing (3'P) reaction and a strand transfer reaction. These enzymatic activities can be measured in vitro with short DNA oligonucleoti...

journal_title：Journal of virological methods

authors： Han YS,Quashie P,Mesplede T,Xu H,Mekhssian K,Fenwick C,Wainberg MA

更新日期：2012-09-01 00:00:00

abstract：:HIV-1 reporter viruses are a critical tool for investigating HIV-1 infection. By having a reporter gene incorporated into the HIV-1 genome, the expressed reporter protein acts as a specific tag, thus enabling specific detection of HIV-1 infected cells. Currently existing HIV-1 reporter viruses utilize reporters for th...

journal_title：Journal of virological methods

authors： Suree N,Koizumi N,Sahakyan A,Shimizu S,An DS

更新日期：2012-07-01 00:00:00

abstract：:The availability of an infectious cDNA clone is a prerequisite for genetic studies on RNA viruses. However, despite important improvement in molecular biology techniques during the last decades, obtaining such clones often remains tedious, time-consuming and rather unpredictable. In the case of potyviruses, cDNA clone...

journal_title：Journal of virological methods

authors： Desbiez C,Chandeysson C,Lecoq H,Moury B

更新日期：2012-07-01 00:00:00

abstract：:The increasing use of the baculovirus expression vector system (BEVS) has generated significant interest into techniques for quantifying baculovirus stocks. One method involves the use of quantitative real-time polymerase chain reaction (PCR). This study investigated simplifying baculovirus sample preparation for quan...

journal_title：Journal of virological methods

authors： George S,Sokolenko S,Aucoin MG

更新日期：2012-06-01 00:00:00

abstract：:Citrus tristeza virus (CTV) is the causal agent of tristeza disease, which is one of the most devastating diseases of citrus crops worldwide. This paper describes a method for the rapid detection and genotyping of naturally spreading CTV isolates. This method uses ELISA or dot-blot immunological tests to detect trees ...

journal_title：Journal of virological methods

authors： Licciardello G,Raspagliesi D,Bar-Joseph M,Catara A

更新日期：2012-05-01 00:00:00

abstract：:Bioinformatics analysis was used to predict potential epitopes of Lyssavirus nucleoprotein and highlighted some distinct differences in the quantity and localization of the epitopes disclosed by epitope analysis of monoclonal antibodies against Lyssavirus nucleoprotein. Bioinformatics analysis showed that the domain c...

journal_title：Journal of virological methods

authors： Xinjun L,Xuejun M,Lihua W,Hao L,Xinxin S,Pengcheng Y,Qing T,Guodong L

更新日期：2012-05-01 00:00:00

abstract：:Activated platelets form transient aggregates with monocytes in circulation and have a half-life of approximately 30-60 min. These complexes are increased in various inflammatory conditions and are an early marker of myocardial infarction. HIV-1 infection is associated with chronic inflammation, and increased CD16⁺ in...

journal_title：Journal of virological methods

authors： Singh MV,Davidson DC,Kiebala M,Maggirwar SB

更新日期：2012-05-01 00:00:00

abstract：:When full-length molecular clones of porcine endogenous retrovirus (PERV)-A and porcine endogenous retrovirus (PERV)-B were passaged on human cells, an increase in the length of the long terminal repeat (LTR) was reported. A 39-bp repeat box in the LTR U3 region was multimerized dynamically upon replication, acting as...

journal_title：Journal of virological methods

authors： Lee YJ,Park SH,Bae EH,Jung YT

更新日期：2012-04-01 00:00:00

abstract：:Group A rotaviruses (RVs) are important pathogens that cause acute, dehydrating gastroenteritis in infants and young children. In this study, a multiplex real-time polymerase chain reaction protocol using primers and TaqMan(®) probes specific for viral VP4 and VP7 genes was evaluated. This assay offers simultaneous ge...

journal_title：Journal of virological methods

authors： Kottaridi C,Spathis AT,Ntova CK,Papaevangelou V,Karakitsos P

更新日期：2012-03-01 00:00:00

abstract：:Two truncated sequences (designated P1 and rHA1) of influenza A virus subtype H5 haemagglutinin (HA) were cloned and expressed in yeast Pichia pastoris (P. pastoris). These polypeptides were used in an indirect recombinant ELISA (rELISA) for detection of H5 antibodies in poultry. Serum samples obtained from broiler ch...

journal_title：Journal of virological methods

authors： Shehata AA,Fiebig P,Sultan H,Hafez M,Liebert UG

更新日期：2012-03-01 00:00:00

abstract：:The genes encoding the Erns and E2 antigen epitopes of classical swine fever virus (CSFV) were expressed as a chimeric protein in Escherichia coli BL21 by pET expression system. The antigenicity of the expressed protein CnC2 was identified by indirect enzyme-linked immunoabsorbant assay (ELISA) and immunoblot with ant...

journal_title：Journal of virological methods

authors： Li X,Wang L,Shi X,Zhao D,Yang J,Yang S,Zhang G

更新日期：2012-03-01 00:00:00

abstract：:A real-time PCR assay based on the TaqMan chemistry was developed for reliable detection and quantitation of the squash leaf curl virus (SLCV) in melon and squash plants. This method was highly specific to SLCV and it was about one thousand times more sensitive than the conventional PCR method. The protocol of the rea...

journal_title：Journal of virological methods

authors： Kuan CP,Huang HC,Chang CC,Lu YL

更新日期：2012-02-01 00:00:00

abstract：:A novel, real-time PCR system for the detection of porcine circovirus type 2 (PCV2) was developed. The system employed Plexor technology and detected 10(8)-10(1) copies per reaction of PCV2 DNA within a recombinant plasmid. The examination of clinical material showed consistent diagnostic sensitivity when samples cont...

journal_title：Journal of virological methods

authors： Vlasakova M,Jackova A,Leskova V,Vilcek S

更新日期：2012-02-01 00:00:00

abstract：:Addition of exogenous peptide sequences on viral capsids is a powerful approach to study the process of viral infection or to retarget viruses toward defined cell types. Until recently, it was not possible to manipulate the genome of mammalian reovirus and this was an obstacle to the addition of exogenous sequence tag...

journal_title：Journal of virological methods

authors： Brochu-Lafontaine V,Lemay G

更新日期：2012-02-01 00:00:00

abstract：:A combined reverse-transcription polymerase chain reaction (RT-PCR) method was developed for the detection and differentiation of wild-type and vaccine strains of the canine distemper virus (CDV). A pair of primers (P1/P2) was used to detect both CDV wild-type strains and vaccines. Another pair (P3/P4) was used to det...

journal_title：Journal of virological methods

authors： Yi L,Cheng S,Xu H,Wang J,Cheng Y,Yang S,Luo B

更新日期：2012-01-01 00:00:00

abstract：:The World Health Organization recommends screening donor blood for HIV in centralized laboratories. This recommendation contributes to quality, but presents specimen transport challenges for resource-limited settings which may be relieved by using dried blood spots (DBS). In sub-Saharan Africa, most countries screen d...

journal_title：Journal of virological methods

authors： Okonji JA,Basavaraju SV,Mwangi J,Shiraishi RW,Odera M,Ouma K,Pitman JP,Marum LH,Ou CY,Zeh C

更新日期：2012-01-01 00:00:00

abstract：:The role of environmental reservoirs in avian influenza virus (AIV) transmission has been investigated during AIV-associated outbreaks. To date, no method has been defined for detection of AIV from mud samples. A procedure using elution and polyethylene glycol (PEG) concentration steps was designed to detect AIV by RT...

journal_title：Journal of virological methods

authors： Deboosere N,Horm SV,Delobel A,Gachet J,Buchy P,Vialette M

更新日期：2012-01-01 00:00:00

abstract：:A real-time reverse transcription-polymerase chain reaction (qRT-PCR) assay was developed for the fast and highly sensitive detection of the sacbrood virus (SBV) genome and applied to honeybee samples. Using plasmid DNA containing a partial SBV genome and diluted serially, as few as 1×10(2)copies/μl (correlation co-ef...

journal_title：Journal of virological methods

authors： Yoo MS,Thi KC,Van Nguyen P,Han SH,Kwon SH,Yoon BS

更新日期：2012-01-01 00:00:00

abstract：:Using a multiplexed LNA-based Taqman assay, RT-digital PCR (RT-dPCR) was performed in a prefabricated microfluidic device that monitored absolute viral load in native and immortalized cell lines, overall precision of detection, and the absolute detection limit of an occult RNA virus GB Virus Type C (GBV-C). RT-dPCR ha...

journal_title：Journal of virological methods

authors： White RA 3rd,Quake SR,Curr K

更新日期：2012-01-01 00:00:00

abstract：:Silver nanoparticles have demonstrated efficient inhibitory activities against human immunodeficiency virus (HIV) and hepatitis B virus (HBV). However, the effects of silver nanoparticles against H1N1 influenza A virus remain unexplored. In this study, the interaction of silver nanoparticles with H1N1 influenza A viru...

journal_title：Journal of virological methods

authors： Xiang DX,Chen Q,Pang L,Zheng CL

更新日期：2011-12-01 00:00:00

abstract：:Reverse transcription loop-mediated isothermal amplification (RT-LAMP) was applied to the detection of equine influenza virus (EIV). Because equine influenza is caused currently by EIV of the H3H8 subtype, the RT-LAMP primer set was designed to target the hemagglutinin gene of this subtype. The detection limit of the ...

journal_title：Journal of virological methods

authors： Nemoto M,Yamanaka T,Bannai H,Tsujimura K,Kondo T,Matsumura T

更新日期：2011-12-01 00:00:00

abstract：:Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed livestock which has a drastic economic impact for affected countries. Although FMDV is distributed worldwide, many regional programs have been effective eradicating this agent. In Argentina, as in many other regions of South America, the comb...

journal_title：Journal of virological methods

authors： Jaworski JP,Compaired D,Trotta M,Perez M,Trono K,Fondevila N

更新日期：2011-12-01 00:00:00

abstract：:The development of a rapid and sensitive system for detecting influenza viruses is a high priority for controlling future epidemics and pandemics. Quantitative real-time PCR is often used for detecting various kinds of viruses; however, it requires more than 2h per run. Detection assays were performed with super high-...

journal_title：Journal of virological methods

authors： Sakurai A,Nomura N,Nanba R,Sinkai T,Iwaki T,Obayashi T,Hashimoto K,Hasegawa M,Sakoda Y,Naito A,Morizane Y,Hosaka M,Tsuboi K,Kida H,Kai A,Shibasaki F

更新日期：2011-12-01 00:00:00

abstract：:Herpes simplex virus type 1 (HSV-1) UL3 protein is a nuclear protein. In this study, the molecular mechanism of the subcellular localization of UL3 was characterized by fluorescence microscopy in living cells. A nuclear localization signal (NLS) and a nuclear export signal (NES) were also identified. UL3 was demonstra...

journal_title：Journal of virological methods

authors： Zheng C,Lin F,Wang S,Xing J

更新日期：2011-11-01 00:00:00

abstract：:The presence of competent vectors in some countries currently free of Rift Valley fever (RVF) and global changes in climate, travel and trade have increased the risk of RVF spreading to new regions and have emphasised the need for accurate and reliable diagnostic tools for early diagnosis during RVF outbreaks. Highly ...

journal_title：Journal of virological methods

authors： Williams R,Ellis CE,Smith SJ,Potgieter CA,Wallace D,Mareledwane VE,Majiwa PA

更新日期：2011-11-01 00:00:00

abstract：:Understanding the ability to survive in an aerosol leads to better understanding of the hazard posed by pathogenic organisms and can inform decisions related to the control and management of disease outbreaks. This basic survival information is sometimes lacking for high priority select agents such as the filoviruses ...

journal_title：Journal of virological methods

authors： Smither SJ,Piercy TJ,Eastaugh L,Steward JA,Lever MS

更新日期：2011-10-01 00:00:00

abstract：:The use of adenovirus type 35 (Ad35) as a vector in vaccine and gene therapy studies is promising due to its broad cell tropism and low seroprevalence in humans. However, to date, a simple and effective system for producing recombinant Ad35 (rAd35) has not been well developed. This report describes a two-plasmid Ad35-...

journal_title：Journal of virological methods

authors： Wu C,Lei X,Wang J,Hung T

更新日期：2011-10-01 00:00:00

abstract：:Enterovirus 71 (EV71), the etiologic agent causes outbreaks with significant mortality in young children in Asia and currently there is no vaccine available. In this study, we report a quantitative enzyme linked immunosorbent assay (Q-ELISA) to determine the concentration of the EV71 VP2 antigen. EV71 virus-like parti...

journal_title：Journal of virological methods

authors： Liu CC,Chang HW,Yang G,Chiang JR,Chow YH,Sai IH,Chang JY,Lin SC,Sia C,Hsiao CH,Chou AH,Chong P

更新日期：2011-09-01 00:00:00